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[Correlation between ERp57 and pregnancy of intrauterine insemination]
Bin Zhang1, Zeng-Hui Bian, Jian-Tang Su
1Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China. zhangbin99011@163.com
Objective:
To investigate the association between the pregnancy rate of intrauterine insemination (IUI) and the ERp57 expression level in donor sperm.
Methods:
Forty-two sperm samples were divided into 3 groups according to the pregnancy rate: Group A (n = 16, pregnancy rate = 0), B (n = 13, pregnancy rate 10%-20%) and C (n = 13, pregnancy rate > or = 20%). The optical density (OD) was normalized to the beta-tubulin band for the evaluation of the ERp57 level.
Results:
OD (ERp57/Tubulin) was 0.95 +/- 0.24 in Group A, 1.33 +/- 0.43 in Group B and 1.33 +/- 0.39 in Group C. The ERp57 expression level was significantly lower in Group A than in B and C (P < 0.05), with no significant differences between the latter two groups.
Conclusion:
The ERp57 expression level in donor sperm could be used as an index to predict the pregnancy rate of IUI and to avoid IUI failure by removing low-level donor sperm.
