Related Experiment Video
Updated: Jun 22, 2026
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
In vivo comparison of hard tissue regeneration with human mesenchymal stem cells processed with either the FICOLL
Sebastian Sauerbier1, Andres Stricker, Jens Kuschnierz
1Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg, Freiburg, Germany. sebastian.sauerbier@uniklinik-freiburg.de
Objective:
To compare new bone formation in maxillary sinus augmentation procedures using biomaterial associated with mesenchymal stem cells (MSCs) separated by two different isolation methods.
Background:
In regenerative medicine open cell concentration systems are only allowed for clinical application under good manufacturing practice conditions.
Methods:
Mononuclear cells, including MSCs, were concentrated with either the synthetic polysaccharide (FICOLL) method (classic open system--control group, n = 6 sinus) or the bone marrow aspirate concentrate (BMAC) method (closed system--test group, n = 12 sinus) and transplanted in combination with biomaterial. A sample of the cells was characterized by their ability to differentiate. After 4.1 months (SD +/- 1.0) bone biopsies were obtained and analyzed.
Results:
The new bone formation in the BMAC group was 19.9% (90% confidence interval [CI], 10.9-29), and in the FICOLL group was 15.5% (90% CI, 8.6-22.4). The 4.4% difference was not significant (90% CI, -4.6-13.5; p = 0.39). MSCs could be differentiated into osteogenic, chondrogenic, and adipogenic lineages.
Conclusion:
MSCs harvested from bone marrow aspirate in combination with bovine bone matrix particles can form lamellar bone and provide a reliable base for dental implants. The closed BMAC system is suited to substitute the open FICOLL system in bone regeneration procedures.
