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Related Experiment Video

Updated: Jun 22, 2026

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves
07:12

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Published on: August 23, 2011

Controlled cyclic stretch bioreactor for tissue-engineered heart valves.

Zeeshan H Syedain1, Robert T Tranquillo

  • 1Department of Chemical Engineering & Materials Science, University of Minnesota, USA.

Biomaterials
|May 29, 2009
PubMed
Summary
This summary is machine-generated.

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This study introduces a novel bioreactor for tissue-engineered heart valves (TEHVs) using controlled cyclic stretching. This method enhances TEHV mechanical properties, offering improved potential for pediatric valve replacement.

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Cardiovascular Research

Background:

  • Tissue-engineered heart valves (TEHVs) offer growth potential for pediatric patients.
  • Current TEHV bioreactors often use pulsed flow, which may not optimally mimic physiological conditions.
  • Improved mechanical properties are crucial for TEHV functionality and longevity.

Purpose of the Study:

  • To develop and evaluate a novel controlled cyclic stretch bioreactor for TEHVs.
  • To assess the impact of this bioreactor on the tensile and compositional properties of TEHVs.
  • To compare the mechanical performance of engineered valves to native pulmonary valve leaflets.

Main Methods:

  • A TEHV was mounted within a latex tube and subjected to cyclic pressurization with culture medium.

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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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Published on: June 14, 2011

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Last Updated: Jun 22, 2026

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves
07:12

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Published on: August 23, 2011

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch
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A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

  • Controlled cyclic stretching was achieved by modulating the latex tube's pressure, dictating TEHV deformation.
  • Fibrin-based TEHVs with human dermal fibroblasts were cultured for three weeks with incrementally increasing strain.
  • Nutrient delivery was supported by concurrent slow perfusion of culture medium through the valve lumen.

    • Main Results:

    • The developed bioreactor successfully induced controlled cyclic stretching in TEHVs.
    • The engineered TEHVs achieved tensile stiffness and anisotropy comparable to native sheep pulmonary valve leaflets.
    • The TEHVs demonstrated similar distensibility under cyclic pulmonary pressures as native pulmonary arteries.

    Conclusions:

    • A novel controlled cyclic stretch bioreactor can significantly enhance the mechanical properties of TEHVs.
    • This bioreactor design shows promise for creating functional TEHVs with native-like mechanical characteristics.
    • The findings support the potential of TEHVs developed with this method for future clinical applications, particularly in pediatric patients.