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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
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Surface plasmon resonance immuno assays - A perspective.

R Gutiérrez-Gallego1, J Bosch, G Such-Sanmartín

  • 1Bioanalysis and Analytical Services Research Group, Neuropsychopharmacology Program, Municipal Institute of Medical Research (IMIM-Hospital del Mar), PRBB, Barcelona, Spain.

Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society
|May 29, 2009
PubMed
Summary

Detecting human growth hormone (GH) doping is difficult due to its natural variations. Measuring GH isoform ratios using surface plasmon resonance (SPR) offers a promising new method for anti-doping efforts.

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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

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Last Updated: Jun 22, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Sports Science

Background:

  • Human growth hormone (GH) is challenging to detect in anti-doping due to its endogenous production, low physiological levels, pulsatile secretion, and isoform heterogeneity.
  • Recombinant GH administration alters endogenous GH isoform ratios, making isoform analysis a key anti-doping strategy.

Purpose of the Study:

  • To explore the potential of surface plasmon resonance (SPR) technology for enhancing human growth hormone (GH) detection in anti-doping.
  • To investigate how SPR can provide insights into GH isoform interactions and improve current immunoassay limitations.

Main Methods:

  • Utilizing surface plasmon resonance (SPR) to monitor biomolecular interactions in a dynamic, label-free setting.
  • Analyzing the potential of SPR for characterizing GH isoform-specific antibody interactions.

Main Results:

  • Conventional immunoassays lack detailed information on antibody-isoform binding kinetics.
  • SPR technology offers a method to gain insights into these specific interactions, potentially improving GH detection.

Conclusions:

  • Surface plasmon resonance (SPR) presents a valuable tool for understanding GH isoform heterogeneity and improving anti-doping immunoassays.
  • SPR may be employed in the future for more accurate and sensitive GH detection in anti-doping surveillance.