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Related Experiment Videos

Development of a pH-controlled fed-batch system for budding yeast.

D Porro1, E Martegani, A Tura

  • 1Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.

Research in Microbiology
|June 1, 1991
PubMed
Summary

A new method uses pH changes to control nutrient feeding in baker's yeast production. This simple approach enhances yeast growth, productivity, and yield in fed-batch systems.

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Area of Science:

  • Biotechnology
  • Industrial Microbiology
  • Bioprocess Engineering

Background:

  • Aerobic fed-batch systems are crucial for baker's yeast manufacturing.
  • Continuous process monitoring is essential for optimizing growth medium addition.
  • Current methods may lack simplicity or efficiency in regulating fed-batch processes.

Purpose of the Study:

  • To develop a simple and effective method for regulating baker's yeast fed-batch processes.
  • To utilize pH changes as a key parameter for controlling nutrient feed.
  • To improve cell density, productivity, and yield in yeast manufacturing.

Main Methods:

  • Development of a novel fed-batch strategy for yeast production.
  • Utilizing real-time pH monitoring to assess yeast metabolic activity.

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  • Implementing a pH-based feedback loop for regulating growth medium flow rate.

    • Main Results:

    • The developed method successfully regulates nutrient addition based on yeast metabolism.
    • High cell densities were achieved in the fed-batch cultures.
    • Significant improvements in both productivity and yield were observed.

    Conclusions:

    • pH monitoring offers a simple and effective parameter for controlling baker's yeast fed-batch processes.
    • The new method enables enhanced performance in terms of cell density, productivity, and yield.
    • This approach provides a valuable tool for optimizing industrial yeast manufacturing.