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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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Perioperative genomic profiles using structure-specific oligonucleotide probes.

Kirk J Hogan1, James K Burmester, Michael D Caldwell

  • 1Department of Anesthesiology, University of Wisconsin School of Medicine and Public Health, B6/319 Clinical Sciences Center, 600 Highland Avenue, Madison, WI 53792, USA. khogan@wiscmail.wisc.edu

Clinical Medicine & Research
|May 29, 2009
PubMed
Summary
This summary is machine-generated.

Perioperative genetic testing reveals significant patient heterogeneity, identifying unknown susceptibilities to complications like drug reactions and sepsis. This new platform detects multiple mutant alleles per patient, improving surgical safety.

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Area of Science:

  • Pharmacogenomics
  • Anesthesiology
  • Medical Genetics

Background:

  • Perioperative complications are often linked to unknown genetic factors.
  • These genetic susceptibilities can lead to adverse events such as drug toxicity, thrombosis, and sepsis.

Purpose of the Study:

  • To design and validate a genetic testing platform for perioperative care.
  • To determine allele frequencies in a surgical patient population.
  • To quantify the number of mutant alleles per patient.

Main Methods:

  • Developed and validated a genetic testing platform for 48 polymorphisms in 22 genes.
  • Utilized structure-specific cleavage of oligonucleotide probes for genotyping.
  • Tested 450 patients undergoing surgery and anesthesia across three Wisconsin medical centers.

Main Results:

  • Confirmed 21,600 genotypes in duplicate.
  • Identified that 376 out of 450 patients (83.6%) had homozygous mutations in at least one gene locus after excluding non-pathogenic polymorphisms.
  • Observed an average of two mutant homozygous loci and ten mutant alleles per patient.

Conclusions:

  • Most patients undergoing surgery exhibit significant genetic heterogeneity.
  • This heterogeneity may not be apparent through family history or routine lab tests.
  • A newly developed genotyping platform can detect these critical genetic variations for improved perioperative management.