Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Scanless temporal focusing enables high-speed three-dimensional quantitative phase microscopy.

Research square·2026

Same author

Evaluation of an Integrated Spectroscopy and Classification Platform for Point-of-Care Core Needle Biopsy Assessment: Performance Characteristics from Ex Vivo Renal Mass Biopsies.

Journal of vascular and interventional radiology : JVIR·2022

Same author

Electrochemically driven optical and SERS immunosensor for the detection of a therapeutic cardiac drug.

RSC advances·2022

Same author

Luminescent Surfaces with Tailored Angular Emission for Compact Dark-Field Imaging Devices.

Nature photonics·2021

Same author

Special Section Guest Editorial: Thirty Years of Multiphoton Microscopy in the Biomedical Sciences.

Journal of biomedical optics·2020

Same author

Plasma membrane temperature gradients and multiple cell permeabilization induced by low peak power density femtosecond lasers.

Biochemistry and biophysics reports·2017

Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026

Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026

Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026

Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026

Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026

Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Interferometric second harmonic generation microscopy.

Siavash Yazdanfar, Lily Laiho, Peter So

|May 29, 2009

Summary

This summary is machine-generated.

We developed a new microscope combining second harmonic generation (SHG) and optical coherence microscopy (OCM) for enhanced imaging. Heterodyne detection in this SHG microscope improves sensitivity for deeper tissue visualization.

More Related Videos

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

Related Experiment Videos

Last Updated: Jun 22, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

Area of Science:

Nonlinear optics
Biomedical imaging
Microscopy

Background:

Second Harmonic Generation (SHG) microscopy offers label-free contrast for biological tissues.
Current SHG detection methods can be limited in sensitivity, restricting imaging depth.
Optical Coherence Microscopy (OCM) provides complementary structural information.

Purpose of the Study:

To introduce a novel microscope integrating heterodyne SHG detection with OCM.
To enhance sensitivity and imaging depth in SHG microscopy.
To enable polarization-sensitive nonlinear susceptibility measurements.

Main Methods:

Development of a microscope employing heterodyne detection for SHG.
Interference of sample SHG with reference crystal SHG.
Integration of OCM for reflectance imaging.
Utilization of dual balanced detectors and polarization-sensitive detection.

Main Results:

The novel microscope successfully combines SHG and OCM.
Heterodyne detection demonstrates potential for improved SHG sensitivity.
Dual balanced detection minimizes source fluctuations.
Polarization-sensitive detection allows nonlinear susceptibility measurement.

Conclusions:

The developed microscope offers advanced capabilities for biological imaging.
Heterodyne detection in SHG microscopy can enhance sensitivity and imaging depth.
The instrument provides multimodal imaging with nonlinear optical contrast and structural information.