Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hydrolysis of Chlorobenzene to Phenol: Dow Process

Hydrolysis of Chlorobenzene to Phenol: Dow Process

Simple aryl halides do not react with nucleophiles under normal conditions. However, the reaction can proceed under drastic conditions involving high temperatures and high pressure to give the substituted products. For example, chlorobenzene is converted to phenol using aqueous sodium hydroxide at 350 °C under high pressure by the Dow process. The reaction follows an elimination-addition mechanism involving a benzyne intermediate. Here, the chloride ion is eliminated to generate the benzyne...

Benzene to Phenol via Cumene: Hock Process

Benzene to Phenol via Cumene: Hock Process

The synthesis of phenol from benzene via cumene and cumene hydroperoxide is called the Hock process. First, a Friedel–Crafts alkylation reaction of benzene with propene gives cumene. Then cumene forms cumene hydroperoxide via a radical chain reaction. In the chain initiation step, the benzylic hydrogen is abstracted to give a benzylic radical. In the chain propagation step, the benzylic radical reacts with an oxygen diradical to form a cumene hydroperoxide radical. The cumene hydroperoxide...

Benzene to 1,4-Cyclohexadiene: Birch Reduction Mechanism

Benzene to 1,4-Cyclohexadiene: Birch Reduction Mechanism

Birch reduction uses solvated electrons as reducing agents. The reaction converts benzene to 1,4-cyclohexadiene. The reaction proceeds by the transfer of a single electron to the ring to form a benzene radical anion. This anion is highly basic—it abstracts a proton from the alcohol to form a cyclohexadienyl radical. Another single electron transfer gives the cyclohexadienyl anion. A proton transfer from the alcohol forms 1,4-cyclohexadiene. Since this reduction occurs via radical anion...

Reactions at the Benzylic Position: Halogenation

Reactions at the Benzylic Position: Halogenation

Benzylic halogenation takes place under conditions that favor radical reactions such as heat, light, or a free radical initiator like peroxide.

Nucleophilic Aromatic Substitution: Elimination–Addition

Nucleophilic Aromatic Substitution: Elimination–Addition

Simple aryl halides do not react with nucleophiles. However, nucleophilic aromatic substitutions can be forced under certain conditions, such as high temperatures or strong bases. The mechanism of substitution under such conditions involves the highly unstable and reactive benzyne intermediate. Benzyne contains equivalent carbon centers at both ends of the triple bond, each of which is equally susceptible to nucleophilic attack. This 50–50 distribution of products is confirmed through isotopic...

NMR Spectroscopy of Benzene Derivatives

NMR Spectroscopy of Benzene Derivatives

Simple unsubstituted benzene has six aromatic protons, all chemically equivalent. Therefore, benzene exhibits only a singlet peak at δ 7.3 ppm in the 1H NMR spectrum. The observed shift is far downfield because the aromatic ring current strongly deshields the protons. Any substitution on the benzene ring makes the aromatic protons nonequivalent, and the protons split each other. The peak is, therefore, no longer a singlet and the splitting pattern and their associated coupling constants depend...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multi-Omics Dissection of the Shared Genetic Architecture Between Sleep Traits and Epilepsy.

Biology·2026

Same author

Chromene-Thiazole Derivatives as Potential SARS-CoV‑2 M<sup>pro</sup> Inhibitors: Synthesis and Computational Studies.

ACS omega·2026

Same author

Targeting NEDD9-SH3 with a Covalent Peptide Controls Endothelial Phenotype.

bioRxiv : the preprint server for biology·2025

Same author

Thermal proteome profiling (TPP) reveals NAMPT as the anti-glioma target of phenanthroindolizidine alkaloid PF403.

Acta pharmaceutica Sinica. B·2025

Same author

Diacytochalasin A:A rearranged cytochalasin from endophytic fungus Diaporthe hongkongensis.

Fitoterapia·2025

Same author

Synthesis, SARS-CoV-2 main protease inhibition, molecular docking and in silico ADME studies of furanochromene-quinoline hydrazone derivatives.

Bioorganic & medicinal chemistry letters·2024

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC

High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC

Published on: February 2, 2024

New benzophenone O-glucoside from Hypericum ellipticum.

Elyse Petrunak¹, Andrew C Kester, Yunbao Liu

¹Department of Chemistry, Susquehanna University, 514 University Avenue, Selinsgrove, PA 17870, USA.

Natural Product Communications

|May 30, 2009

Summary

This summary is machine-generated.

A new benzophenone glucoside from Hypericum ellipticum showed potential in inhibiting central nervous system (CNS) tumor cell proliferation and lipid peroxidation in laboratory tests.

More Related Videos

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

Published on: February 27, 2026

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

Related Experiment Videos

Last Updated: Jun 22, 2026

High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC

High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC

Published on: February 2, 2024

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

Published on: February 27, 2026

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

Area of Science:

Phytochemistry
Natural Products Chemistry
Pharmacology

Background:

The genus Hypericum is known for its diverse phytochemical constituents.
Benzophenone glucosides are a class of compounds with potential biological activities.

Purpose of the Study:

To isolate and characterize new compounds from Hypericum ellipticum.
To evaluate the biological activity of the isolated compounds, specifically their effects on tumor cell proliferation and lipid peroxidation.

Main Methods:

Acetone extraction of aerial parts of Hypericum ellipticum.
Structure elucidation using 2D NMR spectroscopic data.
In vitro assays to assess inhibition of CNS tumor cell line (SF-268) proliferation and lipid peroxidation.

Main Results:

A new acetylated benzophenone glucoside (3 -O-beta-D-3",4",6"-triacetylglucopyranosyl-2,4,5 ,6-tetrahydroxybenzophenone) was isolated.
Catechin and epicatechin were also identified.
The novel benzophenone glucoside demonstrated inhibitory effects on CNS tumor cell proliferation and lipid peroxidation.

Conclusions:

Hypericum ellipticum is a source of novel bioactive benzophenone glucosides.
The isolated compound exhibits promising anti-cancer and anti-oxidative properties.
Further research is warranted to explore its therapeutic potential.