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Expression or secretion of IL-17 in the peripheral blood mononuclear cells from patients with dilated cardiomyopathy

Yanlan Huang1, Weifeng Wu, Yan Wang

  • 1Dept. of Cardiology, the First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.

Acta Cardiologica
|May 30, 2009
PubMed

Insights

Interleukin-17 (IL-17) levels in peripheral blood mononuclear cells (PBMCs) are elevated in dilated cardiomyopathy (DCM) patients. This increased IL-17 expression in PBMCs correlates with DCM severity and impaired heart function.

Area of Science:

  • Immunology
  • Cardiology
  • Molecular Biology

Background:

  • Dilated cardiomyopathy (DCM) is a severe heart condition.
  • The role of interleukin-17 (IL-17) in DCM pathogenesis is not fully understood.

Purpose of the Study:

  • To investigate the relationship between IL-17 levels in plasma and peripheral blood mononuclear cells (PBMCs) and DCM disease severity.

Main Methods:

  • Studied 30 DCM patients and 20 healthy controls.
  • Measured IL-17 protein levels using ELISA and IL-17 mRNA expression via RT-PCR in plasma and PBMCs.
  • Analyzed IL-17 in both unstimulated and phytohemagglutinin (PHA)-stimulated PBMC cultures.

Main Results:

  • Significantly elevated IL-17 protein and mRNA levels were observed in PHA-stimulated PBMCs of DCM patients compared to controls.
  • IL-17 levels in plasma and unstimulated PBMC cultures did not differ significantly.
  • Both IL-17 protein in supernatants and mRNA in PBMCs strongly correlated with DCM patients' heart function.

Conclusions:

  • The ability of PBMCs to express IL-17 is altered in DCM patients.
  • This altered IL-17 expression in PBMCs is a potential biomarker and is strongly associated with heart function in DCM.
Abstract

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