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Electrochemical immunosensor for prostate-specific antigen using self-assembled oligophenylethynylenethiol monolayer

Mi-Ok Namgung1, Seul-Ki Jung, Chan Moon Chung

  • 1Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Republic of Korea.

Ultramicroscopy
|May 30, 2009
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Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.