Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Five genome sequences of bacterial environmental isolates from Gilkey Glacier, Juneau Icefield, Alaska.

Microbiology resource announcements·2026
Same author

Spatial remodeling of bone marrow architecture defines tissue-state signatures of disease activity and therapeutic response in myelodysplastic neoplasms.

Leukemia·2026
Same author

Targeting macrophage ferritin heavy chain mitigates ferroptosis and lung injury in experimental acute respiratory distress syndrome.

Nature communications·2026
Same author

Derivation of elephant induced pluripotent stem cells.

Nature methods·2026
Same author

Shotgun metagenomic dataset of surface microbiomes at a train station in Shinagawa, Tokyo.

BMC genomic data·2026
Same author

Sustained Chlorination of Hospital Surfaces Restructures the Microbiome and Virome and Diversifies Resistance Genes.

Environmental science & technology·2026
Same journal

Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens.

Neuron·2026
Same journal

TGF-β1-induced endothelial transcytosis drives blood-brain barrier leakage during aging.

Neuron·2026
Same journal

Image space opens up for visual neuroscience.

Neuron·2026
Same journal

Septal GLP-1 receptors control alcohol taking and seeking.

Neuron·2026
Same journal

Microglial fitness in moderation: Tuning TREM2 signaling through Ptpn6.

Neuron·2026
Same journal

Human astrocytes keep time with inflammation.

Neuron·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2026

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids
05:45

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Published on: March 29, 2024

Functional and evolutionary insights into human brain development through global transcriptome analysis.

Matthew B Johnson1, Yuka Imamura Kawasawa, Christopher E Mason

  • 1Department of Neurobiology and Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06520, USA.

Neuron
|May 30, 2009
PubMed
Summary
This summary is machine-generated.

This study mapped gene expression in the developing human brain, revealing extensive gene regulation critical for brain development and evolution. These findings illuminate the molecular basis of human cognitive specializations.

More Related Videos

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
07:13

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Published on: November 29, 2024

A Comparative Approach for Quantitative Cell Counting Studies in Widely Different Mammalian Brains
07:14

A Comparative Approach for Quantitative Cell Counting Studies in Widely Different Mammalian Brains

Published on: January 16, 2026

Related Experiment Videos

Last Updated: Jun 22, 2026

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids
05:45

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Published on: March 29, 2024

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
07:13

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Published on: November 29, 2024

A Comparative Approach for Quantitative Cell Counting Studies in Widely Different Mammalian Brains
07:14

A Comparative Approach for Quantitative Cell Counting Studies in Widely Different Mammalian Brains

Published on: January 16, 2026

Area of Science:

  • Neuroscience
  • Genomics
  • Developmental Biology

Background:

  • Understanding human brain development is limited by scarce transcriptome data.
  • Key processes like evolution, formation, and pathological disruption of brain circuits are poorly understood.

Purpose of the Study:

  • To comprehensively analyze the mid-fetal human brain transcriptome.
  • To identify gene expression patterns and regulatory networks in distinct brain regions.
  • To investigate the molecular underpinnings of human brain evolution and cognitive specialization.

Main Methods:

  • Whole-genome, exon-level expression analysis.
  • Analysis of 13 distinct brain regions from the mid-fetal human brain (left and right sides).
  • Identification of differentially regulated genes and coexpression networks.

Main Results:

  • 76% of genes are expressed, with 44% showing differential regulation.
  • Identified specific gene expression, alternative splicing patterns, and coexpression networks linked to brain regions and neurodevelopmental processes.
  • Characterized transcriptional landscapes of prefrontal cortex and speech/language areas, noting global expression symmetry.
  • Found differentially expressed genes are frequently linked to human-specific cis-regulatory element evolution.

Conclusions:

  • The study provides a rich dataset illuminating the molecular complexity of human brain development and evolution.
  • Findings offer insights into the genetic basis of cognitive specializations and human-specific brain evolution.
  • This work establishes a foundation for future research into brain disorders and evolutionary neuroscience.