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Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
Conservative Site-specific Recombination and Phase Variation02:53

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Related Experiment Video

Updated: Jun 22, 2026

Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

New yeast recombineering tools for bacteria.

Robert M Q Shanks1, Daniel E Kadouri, Daniel P MacEachran

  • 1Department of Ophthalmology, University of Pittsburgh Eye Center, PA 15213, USA. shanksrm@upmc.edu

Plasmid
|May 30, 2009
PubMed
Summary

Yeast recombineering streamlines DNA cloning and construct generation. New tools enable efficient DNA manipulation and vector creation for diverse bacterial applications, simplifying complex genetic engineering.

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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
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Published on: December 15, 2012

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Last Updated: Jun 22, 2026

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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
13:06

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Published on: December 15, 2012

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • Microbial Genetics

Background:

  • Recombineering in Saccharomyces cerevisiae is a potent technique for cloning DNA fragments and constructing complex DNA molecules efficiently.
  • Existing methods often require PCR, adding steps and complexity to DNA manipulation.

Purpose of the Study:

  • To introduce novel tools that leverage Saccharomyces cerevisiae's native recombination enzymes for enhanced DNA manipulation.
  • To develop new shuttle vectors for yeast recombineering applicable across various bacterial genera.

Main Methods:

  • Yeast recombineering was employed to create directed nested deletions in a bacteria-yeast shuttle plasmid using single-stranded oligomers, eliminating the need for PCR.
  • Development and characterization of new shuttle vectors designed for broad bacterial replication and yeast recombineering compatibility.

Main Results:

  • Successful generation of directed nested deletions in plasmids without PCR amplification.
  • Creation of versatile shuttle vectors supporting recombineering in diverse bacterial species.
  • Demonstrated utility by generating a pigP deletion mutation in Serratia marcescens.

Conclusions:

  • The developed tools and vectors significantly enhance the efficiency and accessibility of DNA manipulation using yeast recombineering.
  • These advancements facilitate the generation of complex DNA constructs and genetic modifications in various bacterial pathogens.