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Updated: Aug 9, 2026

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Published on: December 12, 2011
Method dependence of interpretation of immunoassay results
U H Stenman1, H Alfthan, U Turpeinen
1Dept Obst Gyn, Helsinki University Central Hospital, FI.
Abstract:
The interpretation of results obtained by immunoassays is affected by many different factors and especially by assay sensitivity and specificity. The introduction of sandwich-type immunometric assays using highly sensitive non-radioactive labels and monoclonal antibodies has greatly improved sensitivity while reducing assay time and simplifying performance. Thus determination of subnormal levels of several hormones has become possible. This approach has also enabled more exact tuning of specificity but at the same time it has introduced the risk of too high selectivity. Hapten assays have also become simpler to perform, but in this case no substantial improvement in assay sensitivity has been achieved.
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