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Updated: Jun 22, 2026

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
Published on: January 14, 2016
Functional analysis of transcription factors in Arabidopsis
Nobutaka Mitsuda1, Masaru Ohme-Takagi
1Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, Higashi, Tsukuba, Japan.
Abstract:
Transcription factors (TFs) regulate the expression of genes at the transcriptional level. Modification of TF activity dynamically alters the transcriptome, which leads to metabolic and phenotypic changes. Thus, functional analysis of TFs using 'omics-based' methodologies is one of the most important areas of the post-genome era. In this mini-review, we present an overview of Arabidopsis TFs and introduce strategies for the functional analysis of plant TFs, which include both traditional and recently developed technologies. These strategies can be assigned to five categories: bioinformatic analysis; analysis of molecular function; expression analysis; phenotype analysis; and network analysis for the description of entire transcriptional regulatory networks.
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