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Ultrasensitive and selective colorimetric DNA detection by nicking endonuclease assisted nanoparticle amplification

Wei Xu1, Xuejia Xue, Tianhu Li

  • 1Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

Angewandte Chemie (International Ed. in English)
|May 30, 2009
PubMed
Abstract

No abstract available in PubMed .

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...