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Modified Yfiler amplification parameters significantly improved Y-short tandem repeat (Y-STR) recovery from degraded skeletal remains, yielding twice as many authentic alleles with minimal increase in errors.

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Area of Science:

  • Forensic Genetics
  • Molecular Biology

Background:

  • Degraded skeletal remains pose challenges for DNA profiling.
  • Y-chromosomal short tandem repeats (Y-STRs) are valuable for identifying male individuals.

Purpose of the Study:

  • To evaluate a modified AmpFlSTR Yfiler PCR Amplification Kit protocol.
  • To assess Y-STR recovery from degraded skeletal samples.

Main Methods:

  • Compared standard vs. modified Yfiler amplification parameters (increased Taq, more cycles).
  • Assessed authentic and drop-in allele recovery using pristine, degraded, and casework DNA samples.

Main Results:

  • Modified parameters doubled authentic allele recovery (62% vs. 31%) with minimal non-authentic alleles (1.36%).
  • Over half of casework samples yielded 9+ reproducible alleles with the modified protocol.

Conclusions:

  • Modified Yfiler amplification enables reproducible Y-STR profiling from challenging degraded and inhibited skeletal remains.
  • This approach enhances forensic identification capabilities.