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Cloning of a serotonin transporter affected by antidepressants
B J Hoffman1, E Mezey, M J Brownstein
1Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.
Science (New York, N.Y.)
|October 25, 1991
Abstract:
A complementary DNA clone for a serotonin (5HT) transporter has been isolated from rat basophilic leukemia cells. The complementary DNA sequence predicts a 653-amino acid protein with 12 to 13 putative transmembrane domains. The 5HT transporter has significant homology to the gamma-aminobutyric acid, dopamine, and norepinephrine transporters. Uptake by CV-1 cells expressing the transporter complementary DNA resembles 5HT uptake by platelets and brain synaptosomes; it is sensitive to antidepressants, amphetamine derivatives, and cocaine.