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Updated: Jun 22, 2026

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
Published on: September 3, 2014
ProSAPiP2, a novel postsynaptic density protein that interacts with ProSAP2/Shank3.
Stefan Liebau1, Christian Proepper, Thomas Schmidt
1Institute of Anatomy and Cell Biology, Albert Einstein Allee 11, 89081 Ulm, Germany.
Researchers identified ProSAP interacting protein 2 (ProSAPiP2), a novel protein interacting with ProSAP2/Shank3. This protein is found in the postsynaptic density (PSD) and may link PSD molecules to the cytoskeleton.
Area of Science:
- Neuroscience
- Cell Biology
- Molecular Biology
Background:
- The postsynaptic density (PSD) is crucial for excitatory synapse function.
- PSD proteins link receptors to the actin cytoskeleton.
- ProSAP/Shank proteins are key scaffolding molecules in the PSD.
Purpose of the Study:
- To characterize a novel ProSAP2/Shank3 interaction partner.
- To investigate the role of this new protein in the PSD.
Main Methods:
- Protein interaction studies using the PDZ domain of ProSAP2/Shank3.
- Expression analysis in the neuronal system.
- Localization studies in dendrites, spines, and PSD.
- Investigation of actin interaction.
Main Results:
- Identified ProSAP interacting protein 2 (ProSAPiP2) as a novel ProSAP2/Shank3 binding partner.
- ProSAPiP2 is highly expressed in neurons and localized to dendrites, spines, and PSD.
- ProSAPiP2 interacts with actin.
Conclusions:
- ProSAPiP2 is a novel PSD protein.
- ProSAPiP2 may mediate the linkage between PSD components and the actin cytoskeleton.
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