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Updated: Jun 22, 2026

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
Published on: April 27, 2018
Ca2+-dependent regulation of mitochondrial dynamics by the Miro-Milton complex
Xingguo Liu1, György Hajnóczky
1Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Abstract:
Calcium oscillations control mitochondrial motility along the microtubules and in turn, support on-demand distribution of mitochondria. However, the mechanism mediating the Ca(2+) effect remained a mystery. Recently, several papers reported on the Ca(2+)-dependent regulation of mitochondrial dynamics by a Miro-Milton complex linking mitochondria to kinesin motors. Both mitochondrial motility and fusion-fission dynamics seem to be sensitive to a Ca(2+)-dependent switch by this complex. Evidence is emerging that calcium signaling through Miro-Milton is central to coordination of the local oxidative metabolism with the energy demands and protection against Ca(2+)-induced cell injury.
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