In vitro integration of human skin dermis with porous cationic hydrogels

Porous poly(DMAA-co-AMTAC) hydrogels, fabricated using the inverted colloid crystal method, were used to observe their integration with human skin. Full thickness human breast skin explants discarded from surgeries were cultured for up to 10days at the air-liquid interface using a Transwell culture system. Cylindrical, disk- or other shaped hydrogels were placed inside the skin explants fitting punctures produced by punch biopsies or scalpels and full section histological analysis of the skin explants with the inserted hydrogel was then performed. In addition, separated hydrogels were cultured up to 7days with human fibroblasts. The results indicate that poly(DMAA-co-AMTAC) hydrogels induce substantial extracellular matrix material deposition, maintain dermal integrity in the contact areas with the skin and permit dermal fibers to integrate into the hydrogel pores. Different types of cells remaining in the explants migrated into the hydrogels pores, including red blood cells. Fibroblasts adhered to and colonized separately cultured hydrogels. We plan to use this type of soft material as an interface to permit skin integration with percutaneous devices in contact with skin.