Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Morphomics: An integral part of systems biology of the human placenta.

Placenta·2015
Same author

Turnover of human villous trophoblast in normal pregnancy: what do we know and what do we need to know?

Placenta·2014
Same author

Cross-sectional data on soft tissue morphometry of the growing hand and fingers of dextral individuals 5-65 years old.

Journal of anatomy·2012
Same author

Fetoplacental angiogenesis in diabetes mellitus.

Placenta·2012
Same author

A quantitative analysis of transcriptionally active syncytiotrophoblast nuclei across human gestation.

Journal of anatomy·2011
Same author

Volumes and numbers of intervillous pores and villous domains in placentas associated with intrauterine growth restriction and/or pre-eclampsia.

Placenta·2010
Same journal

Eucalyptol (1,8-cineole) relaxes umbilical veins in normoglycemic parturients and parturients with gestational diabetes mellitus by modulating ion channels: An ex vivo study.

Placenta·2026
Same journal

Transcriptomic profiling of human placental explants exposed to Plasmodium falciparum reveals activation of tissue-remodeling pathways and signatures associated with the Placenta-Brain Axis.

Placenta·2026
Same journal

Esomeprazole treatment targeting sFLT-1 signaling in preeclampsia improves placental and fetal outcome in mice.

Placenta·2026
Same journal

α7 nicotinic acetylcholine receptor regulates decidual macrophage polarization through TLR4/NF-κB/HIF-1α-mediated glycolytic reprogramming in preeclampsia.

Placenta·2026
Same journal

Progesterone-KISS1 axis impairs amniotic epithelial cell function and promotes premature rupture of membranes.

Placenta·2026
Same journal

Placental iron-driven oxidative imbalance and changes in iron homeostasis in diabetes-in-pregnancy: A cross-sectional analytical study.

Placenta·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2026

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses
09:24

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

Published on: February 18, 2016

Quantifying immunogold localization patterns on electron microscopic thin sections of placenta: recent developments.

T M Mayhew1

  • 1Centre for Integrated Systems Biology and Medicine, School of Biomedical Sciences, E Floor, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK. terry.mayhew@nottingham.ac.uk

Placenta
|June 2, 2009
PubMed
Summary
This summary is machine-generated.

New statistical methods quantify gold-labelled probe distributions in electron microscopy images. These methods compare antigen localization within and between experimental groups, aiding in the analysis of cells, tissues, and organs.

More Related Videos

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
09:33

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

Published on: March 29, 2018

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
08:47

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo

Published on: July 9, 2013

Related Experiment Videos

Last Updated: Jun 22, 2026

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses
09:24

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

Published on: February 18, 2016

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
09:33

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

Published on: March 29, 2018

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
08:47

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo

Published on: July 9, 2013

Area of Science:

  • Quantitative biology
  • Biostatistics
  • Electron microscopy

Background:

  • Advances in electron microscopy enable high-resolution imaging of tissue sections.
  • Colloidal gold-labelled probes are crucial for identifying and localizing target antigens.
  • Quantifying gold particle distributions is essential for comparative analyses.

Purpose of the Study:

  • To present novel statistical methods for quantifying gold particle distributions in electron microscopical images.
  • To enable comparisons of antigen localization within and between experimental groups.
  • To illustrate the application of these methods using placental tissue samples.

Main Methods:

  • Method I: Chi-squared (chi2) analysis for within-group comparisons using labelling density (LD) and relative labelling index (RLI).
  • Method II: Chi2 and contingency table analysis for between-group comparisons, independent of compartment size or magnification.
  • Both methods utilize multistage systematic uniform random sampling and unbiased particle counting.

Main Results:

  • RLI values quantify the degree of preferential labelling, with RLI=1 indicating random distribution.
  • Compartmental chi2 values identify specific sites of within- and between-group differences.
  • The methods were successfully applied to localize caveolin-1 and GLUT1 in human placenta.

Conclusions:

  • The developed statistical methods provide robust tools for quantifying and comparing gold-labelled probe distributions.
  • These methods enhance the analysis of antigen localization in high-resolution microscopy.
  • The findings facilitate a deeper understanding of molecular distributions in biological samples.