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Updated: Jun 22, 2026
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
Published on: June 30, 2016
Sodium caseinate induces secretion of macrophage colony-stimulating factor from neutrophils
E Santiago-Osorio1, L Mora, M Bautista
1Laboratorio de Hematopoyesis y Leucemia, Laboratorio 8, PB, UMIEZ. Campus II. Facultad de Estudios Superiores Zaragoza, UNAM, Batalla 5 de Mayo s/n, Col. E. Oriente, Esquina Fuerte Loreto, Iztapalapa, CP 09230, México, DF., México. edelmiro@servidor.unam.mx
Abstract:
In this work we provide evidence that granulocytes produce macrophage colony-stimulating factor (M-CSF) in the band cell stage and secrete it upon sodium caseinate-mediated differentiation to polymorphonuclear cells. We identified M-CSF in an enriched population of myeloid band cells from murine bone marrow using a chromophore-labeled monoclonal anti-M-CSF antibody. An ELISA assay was then used to detect secreted M-CSF in culture supernatants of enriched band cells differentiated to mature neutrophils using sodium caseinate. Colony formation in vitro by the supernatants from differentiating band cells was blocked by anti-M-CSF, thus suggesting that this factor is the only one responsible for this activity. Our data imply that casein can modulate hematopoiesis possibly via M-CSF production. Finally we discuss the possibility whether this M-CSF in concert with G-CSF could establish a cellular communication network between macrophages and granulocytes allowing them to simultaneously arrive at the inflammatory site.
Insights
Granulocytes produce macrophage colony-stimulating factor (M-CSF) during the band cell stage. This M-CSF is secreted upon differentiation, potentially modulating hematopoiesis and aiding inflammatory site recruitment.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Granulocytes are key immune cells involved in inflammatory responses.
- Macrophage colony-stimulating factor (M-CSF) plays a crucial role in hematopoiesis and immune cell differentiation.
- The specific role of M-CSF production by granulocytes during differentiation is not fully understood.
Purpose of the Study:
- To investigate M-CSF production by granulocytes at the band cell stage.
- To determine if M-CSF is secreted during differentiation into polymorphonuclear cells.
- To explore the potential role of M-CSF in modulating hematopoiesis and cellular communication during inflammation.
Main Methods:
- Identification of M-CSF in enriched myeloid band cells using a chromophore-labeled monoclonal anti-M-CSF antibody.
- Detection of secreted M-CSF in culture supernatants of differentiated band cells via ELISA.
- Assessment of M-CSF's colony-forming activity in vitro and its blockade by anti-M-CSF antibodies.
Main Results:
- M-CSF was identified in myeloid band cells.
- Secreted M-CSF was detected in supernatants from band cells differentiating into neutrophils.
- Anti-M-CSF antibodies blocked the colony-forming activity of these supernatants, confirming M-CSF's role.
- Casein was implied to modulate hematopoiesis through M-CSF production.
Conclusions:
- Granulocytes produce and secrete M-CSF at the band cell stage during differentiation.
- Casein can modulate hematopoiesis potentially via M-CSF production.
- M-CSF, possibly in conjunction with G-CSF, may facilitate communication between macrophages and granulocytes at inflammatory sites.
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