Related Experiment Video
Updated: Jun 22, 2026
08:51
Quantification of Coenzyme A in Cells and Tissues
Published on: September 27, 2019
CoA synthase is in complex with p85alphaPI3K and affects PI3K signaling pathway
Oksana Breus1, Ganna Panasyuk, Ivan T Gout
1Department of Cell Signalling, Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine, Kyiv, Ukraine.
Biochemical and Biophysical Research Communications
|June 2, 2009
Summary
Coenzyme A synthase (CoASy) interacts with PI3K signaling in mitochondria. This discovery reveals a novel link between cellular metabolism and growth factor-dependent signaling pathways.
Area of Science:
- Cellular Biology
- Biochemistry
- Metabolic Regulation
Background:
- Coenzyme A (CoA) derivatives are crucial for cellular metabolism and regulatory processes.
- CoA synthase (CoASy), a mitochondria-associated enzyme, is vital for de novo CoA biosynthesis.
- The interplay between cellular signaling and metabolism is an emerging area of research.
Purpose of the Study:
- To investigate the role of CoA synthase (CoASy) in cellular signaling.
- To determine if CoASy forms functional complexes with key signaling proteins.
- To explore the impact of CoASy on the phosphoinositide 3-kinase (PI3K) signaling pathway.
Main Methods:
- In vivo complex formation assays to detect CoASy and p85alphaPI3K interaction.
- Mitochondrial fractionation to localize PI3K subunits.
- siRNA-mediated knockdown of CoASy to assess effects on PI3K signaling.
Main Results:
- CoASy forms a functional complex with p85alphaPI3K in vivo.
- The CoASy-p85alphaPI3K interaction is regulated by growth factors.
- Both p110alpha and p85alpha subunits of PI3K are found in mitochondria, with p85alpha localized in a complex with CoASy.
- CoASy knockdown significantly alters PI3K signaling pathway activity.
Conclusions:
- CoASy is involved in cellular signaling events, extending beyond its role in CoA biosynthesis.
- Mitochondrial localization of CoASy and its interaction with PI3K suggest a novel regulatory mechanism.
- The CoA biosynthetic pathway plays a role in signal transduction, particularly in response to growth factors.
Related Concept Videos
01:22PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...
02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
01:30Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
01:16Calmodulin-dependent Signaling
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
01:42Phosphoinositides and PIPs
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...