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Updated: Jun 22, 2026
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Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
Published on: May 1, 2020
Phosphorylated eukaryotic translation initiation factor 4 (eIF4E) is elevated in human cancer tissues.
Songqing Fan1, Suresh S Ramalingam, John Kauh
1Department of Hematology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA.
Cancer Biology & Therapy
|June 2, 2009
Summary
Phosphorylated eukaryotic translation initiation factor 4E (p-eIF4E) is significantly elevated in human cancers compared to normal tissues. This finding suggests p-eIF4E plays a role in early tumorigenesis and is a potential therapeutic target.
Area of Science:
- Molecular Biology
- Oncology
- Biochemistry
Background:
- Eukaryotic translation initiation factor 4E (eIF4E) regulates protein synthesis and is implicated in cancer.
- eIF4E phosphorylation by MAPK/Mnk pathways is crucial for its oncogenic activity.
- Expression patterns of phosphorylated eIF4E (p-eIF4E) in human solid tumors remain largely uncharacterized.
Purpose of the Study:
- To investigate the expression levels and patterns of p-eIF4E in a diverse range of human cancer tissues.
- To compare p-eIF4E expression in cancerous tissues versus adjacent normal tissues.
- To explore the correlation between p-eIF4E expression and clinicopathological features, including tumor stage and lymph node metastasis.
Main Methods:
- Utilized three tissue microarrays comprising 380 human cancer cases and 146 adjacent normal tissues.
- Performed immunohistochemical staining to detect p-eIF4E positive staining.
- Analyzed staining intensity and frequency across different cancer types and stages.
Main Results:
- p-eIF4E positive staining was observed in 63.4% of cancers versus 30.1% in normal tissues (p < 0.001).
- No significant difference in p-eIF4E staining was found between cancers with or without lymph node metastasis.
- Early-stage lung, gastric, and colorectal cancers showed significantly higher p-eIF4E staining than late-stage counterparts (p < 0.05).
Conclusions:
- p-eIF4E is significantly upregulated in human cancers, particularly during early stages of tumorigenesis.
- These findings highlight the critical role of p-eIF4E in cancer development.
- p-eIF4E represents a promising therapeutic target for various malignancies.
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