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Related Experiment Video

Updated: Jun 22, 2026

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Published on: June 21, 2024

Flexor tendon tissue engineering: acellularized and reseeded tendon constructs.

Alphonsus K S Chong¹, Jonathan Riboh, R Lane Smith

¹Stanford and Palo Alto, Calif. From the Division of Plastic and Reconstructive Surgery, Stanford University Medical Center; the Bone and Joint Rehabilitation Research and Development Center, Veterans Affairs Palo Alto Health Care System; and the Department of Functional Restoration, Stanford School of Medicine.

Plastic and Reconstructive Surgery

|June 2, 2009

Summary

This summary is machine-generated.

Acellularized flexor tendons maintain strength for tissue engineering. Reseeded scaffolds show slightly reduced stress but retain elastic modulus, offering potential for hand reconstruction grafts.

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Published on: December 10, 2021

Area of Science:

Biomaterials Science
Orthopedic Surgery
Tissue Engineering

Background:

Flexor tendon tissue engineering demands scaffolds with sufficient strength and biocompatibility.
The biomechanical properties of acellularized and reseeded flexor tendon scaffolds remain largely uncharacterized.
This study investigates alterations in biomechanical properties following acellularization and reseeding treatments.

Purpose of the Study:

To evaluate the biomechanical integrity of acellularized and tenocyte-reseeded rabbit flexor tendons.
To determine the suitability of these modified tendons as scaffolds for tissue engineering applications.
To assess the potential of this method for creating intrasynovial graft material for hand reconstruction.

Main Methods:

Rabbit flexor tendons underwent acellularization using freeze-thaw cycles, trypsin, and Triton-X.
Histological analysis and cell recovery attempts confirmed complete acellularization.
Acellularized tendons were reseeded with tenocytes, followed by tensile testing to compare ultimate stress and elastic modulus against control tendons.

Main Results:

The acellularization protocol effectively removed cells from flexor tendons.
Reseeding with tenocytes resulted in surface cell adhesion.
Acellularized tendons exhibited biomechanical properties comparable to native tendons; reseeded tendons maintained elastic modulus but showed a significant decrease in ultimate stress.

Conclusions:

Acellularized flexor tendons serve as promising high-strength scaffolds for tendon tissue engineering.
The acellularization and reseeding technique offers a viable strategy for generating intrasynovial graft material.
This approach holds potential for advancing reconstructive surgery in hand injuries.