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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 22, 2026

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

Cavity-enhanced emission from a dye-coated microsphere.

Perry Schiro, Alfred Kwok

    Optics Express
    |June 2, 2009
    PubMed
    Summary

    Whispering gallery modes were observed in dye-coated polystyrene beads. This study used specialized lasers to isolate single beads for analysis without causing damage, advancing microparticle research.

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Biophysics

    Background:

    • Whispering gallery modes (WGMs) are confined light waves that can be generated in dielectric microstructures.
    • Dye-coated microparticles are used in various sensing and imaging applications.
    • Studying single microparticles in suspension requires precise isolation techniques to avoid damage and ensure accurate measurements.

    Purpose of the Study:

    • To observe and characterize whispering gallery modes in the inelastic emission of a single, dye-coated polystyrene microparticle.
    • To demonstrate a method for isolating and studying individual dye-coated beads in colloidal suspension without inducing damage.
    • To explore the potential of WGMs in dye-doped microparticles for advanced optical applications.

    Main Methods:

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    Published on: March 13, 2013

    Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications
    08:06

    Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications

    Published on: June 2, 2017

    Related Experiment Videos

    Last Updated: Jun 22, 2026

    Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
    12:57

    Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

    Published on: October 13, 2017

    Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
    08:12

    Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

    Published on: March 13, 2013

    Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications
    08:06

    Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications

    Published on: June 2, 2017

  • Utilized a 9.8 micrometer polystyrene bead coated with a monolayer of AlexaFluor 488 dye.
  • Employed a dual-laser system: a near-infrared (near-IR) trapping laser for isolation and an Argon ion (Ar+) excitation laser for optical stimulation.
  • Studied the inelastic emission from the single, isolated dye-coated bead within a colloidal suspension.

    • Main Results:

    • Successfully observed whispering gallery modes in the inelastic emission spectrum of the AlexaFluor 488 dye-coated polystyrene bead.
    • Demonstrated the capability to isolate and study a single microparticle using distinct trapping and excitation lasers, preventing bead damage.
    • Collected data on the optical properties of the dye-coated bead through its inelastic emission.

    Conclusions:

    • The observation of WGMs in dye-coated microparticles opens possibilities for novel optical sensing and light-emitting devices.
    • The employed dual-laser technique provides a robust method for non-damaging, single-particle analysis in colloidal systems.
    • Further research can explore tuning WGM properties by altering dye type, bead size, or environmental conditions.