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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...

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Related Experiment Video

Updated: Jun 22, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Supercritical angle fluorescence (SAF) microscopy.

Thomas Ruckstuhl, Dorinel Verdes

    Optics Express
    |June 2, 2009
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel confocal microscope for detecting surface fluorescence and analyzing biochips. It simultaneously measures surface-bound and unbound analytes using two distinct fluorescence collection modes.

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    Area of Science:

    • Microscopy
    • Biophotonics
    • Analytical Chemistry

    Background:

    • Confocal microscopy is vital for high-resolution imaging.
    • Detecting surface-generated fluorescence is crucial for biochip analysis.
    • Simultaneous measurement of bound and unbound analytes presents a challenge.

    Purpose of the Study:

    • To present a new confocal microscope design for enhanced surface fluorescence detection.
    • To enable high-resolution imaging and large biochip readout.
    • To achieve simultaneous measurement of surface-bound and unbound fluorescent analytes.

    Main Methods:

    • Development of a novel confocal microscope.
    • Implementation of two separated fluorescence emission collection paths.
    • Utilizing supercritical angle fluorescence (SAF) for interface confinement.
    • Collecting low-angle emissions for deeper analyte solution analysis.

    Main Results:

    • The instrument achieves high-resolution imaging and biochip readout.
    • Separated collection paths allow for distinct fluorescence detection modes.
    • SAF collection strictly confines the detection volume to the interface.
    • Low-angle collection gathers data from the aqueous analyte solution.

    Conclusions:

    • The new confocal microscope design enables simultaneous detection of surface-bound and unbound fluorescent analytes.
    • This dual-mode detection provides comprehensive information from biochip surfaces and solutions.
    • The instrument offers a significant advancement in bioanalytical imaging and detection.