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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
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Related Experiment Video

Updated: Jun 22, 2026

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

Bipolar absolute differential confocal approach to higher spatial resolution.

Weiqian Zhao, Jiubin Tan, Lirong Qiu

    Optics Express
    |June 2, 2009
    PubMed
    Summary

    We developed a superresolution microscopy technique for ultraprecision 3D microstructure measurement. This high spatial resolution approach achieves sub-micrometer lateral and nanometer axial resolution, advancing metrology capabilities.

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    Last Updated: Jun 22, 2026

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    Published on: May 19, 2022

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    Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
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    Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

    Published on: August 11, 2020

    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Metrology

    Background:

    • Achieving high spatial resolution in 3D microstructure measurement is crucial for advanced manufacturing and scientific research.
    • Conventional microscopy techniques often face limitations in simultaneously achieving both high lateral and axial resolution.

    Purpose of the Study:

    • To propose and validate a novel high spatial resolution bipolar absolute differential confocal approach.
    • To enhance the precision of three-dimensional (3D) microstructure measurements.

    Main Methods:

    • Utilizing a superresolution pupil filtering technique for enhanced lateral optical resolution.
    • Employing a differential confocal microscopy technique for nanometer-level axial resolution.
    • Implementing a shaped annular beam differential confocal microscopy system for experimental validation.

    Main Results:

    • Demonstrated a lateral resolution better than 0.2 µm.
    • Achieved an axial resolution better than 2 nm.
    • Validated the approach using a shaped annular beam differential confocal microscopy system under specific optical conditions (λ=632.8 nm, ε=0.5, µM=6.95, NA=0.85).

    Conclusions:

    • The proposed bipolar absolute differential confocal approach enables ultraprecision 3D microstructure measurement.
    • The experimental results confirm the system's capability to surpass conventional resolution limits.
    • This technique offers a significant advancement in high-resolution metrology.