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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

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Related Experiment Video

Updated: Jun 22, 2026

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
12:56

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

Published on: October 17, 2010

Optical coherence tomography using a continuous-wave, high-power, Raman continuum light source.

Pei-Lin Hsiung, Yu Chen, Tony Ko

    Optics Express
    |June 2, 2009
    PubMed
    Summary
    This summary is machine-generated.

    A new all-fiber Raman light source offers high performance for ultrahigh resolution optical coherence tomography (OCT) imaging. This compact, robust source enables high-speed OCT with sub-5 micrometer axial resolution.

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    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

    Published on: August 4, 2018

    Related Experiment Videos

    Last Updated: Jun 22, 2026

    Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
    12:56

    Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

    Published on: October 17, 2010

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
    12:22

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

    Published on: August 4, 2018

    Area of Science:

    • Biomedical Optics
    • Laser Physics
    • Medical Imaging

    Background:

    • High-performance light sources are essential for advanced optical coherence tomography (OCT) imaging.
    • Existing OCT light sources often face limitations in bandwidth, output power, or system complexity.

    Purpose of the Study:

    • To demonstrate a novel, high-performance light source optimized for ultrahigh resolution OCT.
    • To achieve broad bandwidth and high output power for improved OCT imaging capabilities.

    Main Methods:

    • Development of an all-fiber Raman light source.
    • Utilizing a continuous-wave Yb-fiber laser-pumped microstructure fiber.
    • Characterization of bandwidth, output power, and coherence length.

    Main Results:

    • Achieved a broad bandwidth of 140 nm centered at 1300 nm.
    • Generated high output power of 330 mW.
    • Demonstrated a compact, robust, and turnkey light source requiring no optical alignment.

    Conclusions:

    • The developed Raman light source meets critical requirements for high-speed, ultrahigh resolution OCT.
    • Successfully demonstrated in vivo, ultrahigh resolution, high-speed, time-domain OCT imaging with <5 micrometer axial resolution.