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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jun 22, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

High sensitivity protein assays on microarray silicon slides.

Marina Cretich¹, Gabriele di Carlo, Renato Longhi

¹Istituto di Chimica del Riconoscimento Molecolare, CNR, 20131 Milano, Italy.

Analytical Chemistry

|June 3, 2009

Summary

This summary is machine-generated.

This study enhances microarray sensitivity using a silicon substrate with silicon oxide and a novel polymer coating. This innovation boosts fluorescence signals 5-10 fold for improved diagnostic applications.

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Last Updated: Jun 22, 2026

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Published on: January 7, 2019

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Area of Science:

Materials Science
Biotechnology
Surface Chemistry

Background:

Microarray technology relies on sensitive detection methods.
Current microarray substrates can limit fluorescence signal intensity.
Optimizing substrate and surface chemistry is crucial for enhanced performance.

Purpose of the Study:

To improve microarray sensitivity through a novel substrate design.
To enhance fluorescence signal detection in microarray experiments.
To develop a cost-effective and scalable solution for diagnostic applications.

Main Methods:

Utilized crystalline silicon substrates coated with thermal silicon oxide (SiO(2)).
Functionalized the SiO(2) surface with a specific copolymer (copoly(DMA-NAS-MAPS)).
Evaluated substrate performance in protein and peptide microarrays using fluorescence labeling and confocal laser scanning.

Main Results:

The optimized silicon oxide layer enhanced fluorescence via constructive interference.
The polymeric coating provided optimal binding specificity and a high signal-to-noise ratio.
Achieved a 5-10 fold increase in fluorescence signals compared to standard glass slides.

Conclusions:

Combining an optimized reflective substrate with high-performance surface chemistry significantly boosts microarray sensitivity.
The developed techniques are simple, inexpensive, and suitable for mass production.
The coated silicon slides show great potential for clinical diagnostic applications.