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Updated: Jun 22, 2026

Mitochondrial Transformation in Baker's Yeast to Study Translation and Respiratory Complex Assembly
Published on: June 7, 2024
Chimeric types of chromosome X in bottom-fermenting yeasts
T Ogata1, M Izumikawa, H Tadami
1Brewing Research & Development Laboratory, Asahi Breweries, Ltd, 1-1-21, Midori, Moriya, Ibaraki 302-0106, Japan. tomoo.ogata@asahibeer.co.jp
Aim:
To determine the structure of the chimeric chromosome X of bottom-fermenting yeasts.
Methods And Results:
Eight cosmid clones carrying DNA from chromosome X of bottom-fermenting yeasts were selected by end-sequencing. Four of the cosmid clones had Saccharomyces cerevisiae (SC)-type and Saccharomyces bayanus (SB)-type chimeric ends, two had SC-type ends and two had SB-type ends. Sequencing revealed that the bottom-fermenting yeast strains in this study had four types of chromosome X: SC-SC, SC-SB, SB-SC and SB-SB. The translocation site in the chimeric chromosome is conserved among bottom-fermenting yeast strains, and was created by homologous recombination within a region of high sequence identity between the SC-type sequence and the SB-type sequence.
Conclusions:
Existing bottom-fermenting yeast strains share a common ancestor in which the chimeric chromosome X was generated.
Significance And Impact Of The Study:
The knowledge gained in this study sheds light on the evolution of bottom-fermenting yeasts.
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