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Updated: Jun 22, 2026
Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
Published on: April 6, 2022
Modelling the photosensitization-based inactivation of Bacillus cereus
Y Le Marc1, I Buchovec, S M George
1Institute of Food Research, Norwich Research Park, Norwich, UK. yvan.lemarc@bbsrc.ac.uk
Aims:
To study and to develop a model for the photo-destruction of the foodborne pathogen Bacillus cereus, initially treated with a precursor of endogenous photosensitizers (5-aminolevulinic acid, ALA).
Materials And Methods:
The cells were incubated in the presence of ALA (3 or 7.5 mmol l(-1)) for incubation times ranging from 2 to 60 min, inoculated onto the surface of LB Agar plates and submitted to light irradiation. The Weibull model was used to describe the survival curves of B. cereus. Quadratic equations were used to describe the effects of ALA concentration and incubation time on the Weibull model parameters.
Results:
ALA-based photosensitization proved to be an effective tool for inactivation of B. cereus. The decrease in viable counts observed after 20 min of irradiation, ranged from 4 to 6 log CFU g(-1).
Conclusions:
The developed model proved to be a parsimonious and robust solution to describe the observed data.
Significance And Impact Of The Study:
The study demonstrates the effectiveness of photosensitization on B. cereus on agar plates. The model developed may be useful to optimize inactivation treatments by photosensitization.
