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A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

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Published on: December 25, 2015

High reproducibility of ammonia-oxidizing bacterial communities in parallel sequential batch reactors.

L Wittebolle¹, N Van Vooren, W Verstraete

¹Laboratory of Microbial Ecology & Technology, Ghent University, Gent, Belgium.

Journal of Applied Microbiology

|June 3, 2009

Summary

Ammonia-oxidizing bacterial communities in replicate bioreactors co-evolved over time. After an initial phase, these bacterial communities demonstrated high functional stability and low dynamics during complete nitrification.

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Area of Science:

Environmental microbiology
Wastewater treatment
Nitrification processes

Background:

Ammonia-oxidizing bacteria (AOB) are crucial for nitrification in wastewater treatment.
Understanding AOB community dynamics is essential for optimizing bioreactor performance.

Purpose of the Study:

To investigate the co-evolution or divergence of AOB communities in replicate nitrifying bioreactors.
To assess the stability and dynamics of AOB communities during complete nitrification.

Main Methods:

Three parallel sequential batch reactors (SBRs) were operated for 134 days.
Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze AOB community structure.
AOB community dynamics were quantified using rate of change values (Delta(t(week))).

Main Results:

PCR-DGGE confirmed co-evolution of AOB communities in replicate bioreactors.
AOB community dynamics were high (12-22%) during start-up with temporary nitrification decreases.
Low AOB community dynamics (<5%) and high functional stability were observed during complete nitrification.

Conclusions:

Nitrifying bioreactors exhibit high functional stability and low AOB community dynamics after the start-up phase.
Deterministic factors, not stochastic ones, drive AOB community co-evolution in replicate systems.
DGGE pattern analysis is a reliable method for assessing bacterial community functionality and reproducibility.