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Updated: Jun 22, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Identification of a novel protein binding to hepatitis C virus core protein
Yun-Ru Chen1, Tian-Yan Chen, Shu-Lin Zhang
1The First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Background:
Hepatitis C virus (HCV) core protein is a multi-functional viral protein that interacts with several target proteins of both viral and cellular origin.
Aim And Methods:
To gain insight into the mechanism of action of HCV core protein, we used a yeast two-hybrid system to identify the core protein-interacting cellular targets.
Results:
A cDNA clone encoding an aspartoacylase was obtained, termed aspartoacylase 3 (ACY3). Interaction between ACY3 and HCV core protein was verified using a co-immunoprecipitation assay in vitro, and a mammalian two-hybrid system in vivo. Fluorescence microscopy showed green fluorescence protein-fused ACY3 localized in the cytoplasm.
Conclusion:
Our data suggest that ACY3 is an HCV core binding protein, which may play a role in the development of HCV-associated diseases.
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