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Updated: Jun 22, 2026
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018
Genotypic characterization of Pseudomonas aeruginosa isolated from human and animal sources in Egypt
K M Osman1, M S Alabady, N S S M Ata
1Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt. s_mougy@hotmail.com
Abstract:
Two different techniques for the molecular typing of Pseudomonas aeruginosa were used to study the epidemiology of P. aeruginosa strains. Colonization with P. aeruginosa was studied by taking samples of human origin collected from urine, sputum samples of patients suffering from lung manifestations and patients exposed to third-degree burns. In addition, samples of animal origin were collected from mastitic milk and lung tissues of slaughtered calves and from the internal organs of diseased chickens. Typing of 18 isolates was performed by random amplified polymorphic DNA analysis and amplified fragment length polymorphism analysis. Computer-aided cluster analysis indicated that similar groups of related isolates were obtained by each method.
Insights
Molecular typing of Pseudomonas aeruginosa using two DNA-based methods revealed similar groupings of strains from human and animal origins. This helps understand the epidemiology of P. aeruginosa infections.
Area of Science:
- Microbiology
- Epidemiology
- Molecular Biology
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen causing infections in humans and animals.
- Understanding the molecular epidemiology of P. aeruginosa is crucial for infection control.
Purpose of the Study:
- To compare two molecular typing techniques for Pseudomonas aeruginosa.
- To investigate the epidemiological relationship of P. aeruginosa strains from various sources.
Main Methods:
- Molecular typing of 18 P. aeruginosa isolates using random amplified polymorphic DNA (RAPD) analysis.
- Amplified fragment length polymorphism (AFLP) analysis was also performed.
- Computer-aided cluster analysis was used to group related isolates.
Main Results:
- Both RAPD and AFLP analyses generated similar groupings of P. aeruginosa strains.
- Isolates from human and animal sources showed relatedness based on molecular typing.
- The study successfully differentiated P. aeruginosa strains based on their genetic profiles.
Conclusions:
- Random amplified polymorphic DNA and amplified fragment length polymorphism are effective methods for P. aeruginosa molecular typing.
- The findings suggest potential transmission or shared sources of P. aeruginosa between human and animal populations.
- Further epidemiological studies can benefit from these molecular typing approaches.
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