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Updated: Jun 22, 2026
A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
Published on: March 9, 2016
An alternative method for DNA extraction and PCR identification of Entamoeba histolytica and E. dispar in fecal
E N Vianna1, J O Costa, C K S Santos
1Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627-31270-901, Belo Horizonte, Minas Gerais, Brazil.
Abstract:
Since it is known that Entamoeba dispar is non-pathogenic and morphologically similar to E. histolytica, there are many targets used in PCR for differentiating these species. However, obtaining high quality DNA from fecal samples is fundamental for PCR. Most methods are laborious or use kits that make diagnosis expensive. In the present work, a new simple, fast and cheap technique of DNA extraction from fecal samples was combined with a PCR for an episomal target in order to identify E. histolytica and E. dispar in feces.
