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Related Experiment Video

Updated: Jun 22, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Sialic acid in human testis and changes with aging.

G Gheri¹, D Vichi, G D Zappoli Thyrion

¹Department of Anatomy Histology and Forensic Medicine, University of Florence, Policlinic of Careggi, Viale Morgagni 85, 50134 Florence, Italy.

Reproduction, Fertility, and Development

|June 3, 2009

Summary

This summary is machine-generated.

Changes in human testis sialoderivatives occur with aging. These sialic acid variations in the aging testis suggest their importance in male reproductive function and involution.

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Published on: July 14, 2017

Area of Science:

Reproductive Biology
Glycobiology
Human Aging Research

Background:

Sialoderivatives, a class of glycoconjugates, play crucial roles in cellular function.
The distribution and structural changes of sialoderivatives in the aging human testis are not well understood.
Understanding these changes is vital for comprehending male reproductive health and age-related involution.

Purpose of the Study:

To investigate the distribution and structural characteristics of sialoderivatives in the human testis.
To compare sialoderivative patterns between young adult and elderly men.
To elucidate the role of sialoderivatives in testicular aging.

Main Methods:

Utilized orchidectomy specimens from two age groups (18-30 and 70-93 years).
Employed digoxigenin-labelled lectins: Maackia amurensis II lectin (MAA), Sambucus nigra agglutinin (SNA), and Arachis hypogaea lectin (PNA).
Performed enzymatic and chemical treatments (neuraminidase, oxidation) to characterize sialic acid linkages and structures.

Main Results:

Sialic acids linked alpha-2,3 and alpha-2,6 to galactose/N-acetyl-D-galactosamine (Gal/GalNAc) were prevalent in the interstitial tissue and lamina propria of older men (Group 2).
Alpha-2,6 linked sialic acids were less common in younger men (Group 1).
Arachis hypogaea lectin (PNA) indicated structural changes and loss of specific sialic acid linkages with aging.

Conclusions:

Aging is associated with significant alterations in sialoderivative metabolism and distribution within the human testis.
These age-related changes in sialoderivatives correlate with morphofunctional changes in testicular components.
Sialoderivatives are implicated in the functionality of the mature human testis and its age-related involutional processes.