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Related Experiment Video

Updated: Jun 22, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Variable wavelength surface plasmon resonance (SPR) in biosensing.

Nyeon-Sik Eum1, Do-Eok Kim, Se-Hyuk Yeom

  • 1Department of Agriculture and Biological Engineering, Purdue University, 225 South University Street, West Lafayette, IN 47907, USA.

Bio Systems
|June 3, 2009
PubMed
Summary

This study introduces a novel variable wavelength surface plasmon resonance (SPR) sensor for detecting microscopic refractive index changes. The SPR sensor successfully detected Salmonella antigen-antibody reactions and penicillinase-penicillin interactions.

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Correction: Kim et al. Effects of Nursing Simulation Using Mixed Reality: A Scoping Review. <i>Healthcare</i> 2021, <i>9</i>, 947.

Healthcare (Basel, Switzerland)·2021

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biosensing Technology

Background:

  • Surface Plasmon Resonance (SPR) is a label-free optical technique widely used for detecting biomolecular interactions.
  • Traditional SPR sensors often operate at a fixed wavelength, limiting their sensitivity and dynamic range.
  • Developing variable wavelength SPR sensors can enhance detection capabilities for various analytes.

Purpose of the Study:

  • To fabricate and characterize a novel variable wavelength surface plasmon resonance (SPR) sensor.
  • To demonstrate the sensor's capability in detecting antigen-antibody reactions (Salmonella) and enzyme-substrate interactions (penicillinase-penicillin).
  • To quantify the shifts in resonant wavelength as a measure of molecular interactions.

Main Methods:

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  • Fabrication of a variable wavelength SPR sensor utilizing a white light source, multimode optical fiber, and sensor chip.
  • Immobilization of Salmonella antibody and penicillinase onto the sensor chip surfaces.
  • Injection of Salmonella antigen solution and penicillin solution to induce reactions and measure resonant wavelength shifts using an optical spectrum analyzer.

    • Main Results:

    • The SPR sensor detected a resonant wavelength shift of approximately 0.26 nm/min during the Salmonella antibody-antigen reaction.
    • The sensor also detected a wavelength shift of 0.8 nm/min over 15 minutes for the penicillinase-penicillin interaction.
    • These results demonstrate the sensor's ability to measure changes in microscopic refractive index caused by specific molecular binding events.

    Conclusions:

    • The developed variable wavelength SPR sensor is effective for label-free detection of biomolecular interactions.
    • The sensor shows promise for sensitive and quantitative analysis of both immunological and enzymatic reactions.
    • This technology offers a versatile platform for biosensing applications in diagnostics and research.