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Related Experiment Video

Updated: Jun 22, 2026

Measuring Caenorhabditis elegans Life Span on Solid Media
12:49

Measuring Caenorhabditis elegans Life Span on Solid Media

Published on: May 12, 2009

Measuring Caenorhabditis elegans life span on solid media.

George L Sutphin1, Matt Kaeberlein

  • 1Department of Pathology, University of Washington, Seattle, WA, USA.

Journal of Visualized Experiments : Jove
|June 3, 2009
PubMed
Summary
This summary is machine-generated.

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This study details a method for measuring the lifespan of the nematode Caenorhabditis elegans. This protocol aids in understanding aging and testing interventions in aging research.

Area of Science:

  • Gerontology
  • Developmental Biology
  • Genetics

Background:

  • Aging is a natural degenerative process affecting all biological systems, leading to mortality.
  • The nematode Caenorhabditis elegans is a key model organism for studying aging due to its short lifespan and genetic tractability.
  • Life span, defined by responsiveness to stimuli, is a critical metric for assessing the rate of aging.

Purpose of the Study:

  • To present a standardized protocol for measuring nematode lifespan.
  • To provide a adaptable method for aging studies in Caenorhabditis elegans.
  • To facilitate research on aging biology and potential interventions.

Main Methods:

  • Utilizing solid nematode growth media plates for nematode maintenance.
  • Feeding nematodes a diet of UV-killed bacteria.

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Surveying Low-Cost Methods to Measure Lifespan and Healthspan in Caenorhabditis elegans
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Last Updated: Jun 22, 2026

Measuring Caenorhabditis elegans Life Span on Solid Media
12:49

Measuring Caenorhabditis elegans Life Span on Solid Media

Published on: May 12, 2009

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates
12:23

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates

Published on: March 18, 2011

Surveying Low-Cost Methods to Measure Lifespan and Healthspan in Caenorhabditis elegans
10:08

Surveying Low-Cost Methods to Measure Lifespan and Healthspan in Caenorhabditis elegans

Published on: May 18, 2022

  • Establishing a generalized protocol for lifespan measurement adaptable to various conditions.

    • Main Results:

    • A reproducible method for quantifying nematode lifespan was established.
    • The protocol allows for lifespan assays under diverse conditions, including different diets and genetic manipulations.
    • This method supports the study of aging in Caenorhabditis elegans.

    Conclusions:

    • The presented protocol offers a robust and adaptable approach to aging research in Caenorhabditis elegans.
    • This standardized method can accelerate the discovery of factors influencing aging and lifespan.
    • The protocol's flexibility supports a wide range of experimental designs in gerontology.