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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Computed Tomography01:10

Computed Tomography

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Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Imaging Biological Samples with Optical Microscopy

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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Electron Microscope Tomography and Single-particle Reconstruction

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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Full range polarization-sensitive Fourier domain optical coherence tomography.

Jun Zhang, Woonggyu Jung, J Nelson

    Optics Express
    |June 3, 2009
    PubMed
    Summary
    This summary is machine-generated.

    A novel swept source Fourier domain optical coherence tomography (FDOCT) system accurately measures polarization states in biological tissues. This advanced optical coherence tomography (OCT) method overcomes limitations of previous systems for detailed imaging.

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    Area of Science:

    • Biomedical Optics
    • Optical Coherence Tomography
    • Polarization Imaging

    Background:

    • Conventional time domain OCT (TDOCT) and spectrometer-based FDOCT systems have limitations due to wavelength-dependent input polarization states.
    • Accurate characterization of light polarization is crucial for advanced tissue imaging and analysis.

    Purpose of the Study:

    • To develop a swept source-based polarization-sensitive Fourier domain optical coherence tomography (FDOCT) system.
    • To enable the acquisition of Stokes vectors, polarization diversity intensity, and birefringence images in biological tissues.

    Main Methods:

    • Reconstruction of both amplitude and phase terms of the interference signal.
    • Determination of Stokes vectors by processing analytical complex fringe signals from two perpendicular polarization-detection channels.
    • Accurate setting of input polarization states, overcoming wavelength dependency issues.

    Main Results:

    • The developed swept source FDOCT system successfully acquired Stokes vectors and polarization-sensitive images.
    • Birefringence and polarization diversity intensity images were obtained from biological tissues.
    • The system demonstrated accurate control over input polarization states.

    Conclusions:

    • The novel swept source FDOCT system provides a robust platform for polarization-sensitive imaging in biological tissues.
    • This technology overcomes key limitations of existing OCT systems, enabling more comprehensive tissue characterization.