Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum BP180 and BP230 autoantibodies in Alzheimer's disease: diagnostic and cognitive associations in a retrospective study.

Frontiers in aging neuroscience·2026
Same author

Connective tissue diseases combined with chronic myelomonocytic leukemia and myelofibrosis: a case report and literature review.

Frontiers in immunology·2026
Same author

Evaluation of the Effectiveness of Longitudinal Incision for Endoscopic Submucosal Excavation of Gastric Subepithelial Lesions.

Journal of visualized experiments : JoVE·2026
Same author

Mechanistic insights into cannabidiol-mediated TrkB activation via FRS2 interaction in attenuating Alzheimer's disease pathology and cognitive impairment.

Molecular psychiatry·2026
Same author

A novel cannabidiol-derived small molecule ameliorates lower urinary tract dysfunction in mice with spinal cord injury via suppressing neuroinflammation.

Life sciences·2026
Same author

Successful Treatment of Pityriasis Rubra Pilaris With Deucravacitinib.

JAMA dermatology·2026

Related Experiment Video

Updated: Jun 22, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Nanoparticle-mediated gene delivery.

Sha Jin1, John C Leach, Kaiming Ye

  • 1College of Engineering, University of Arkansas, Fayetteville, AR, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2009
PubMed
Summary
This summary is machine-generated.

Nonviral gene delivery offers a safer alternative to viral methods, enabling targeted gene therapy with functionalized nanoparticles like superparamagnetic iron oxide and silica nanoparticles.

More Related Videos

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Related Experiment Videos

Last Updated: Jun 22, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Gene Therapy

Background:

  • Nonviral gene delivery is gaining attention as a safer alternative to viral vectors.
  • While less efficient in DNA transfection, nonviral vectors offer advantages like lower immunogenicity and no risk of viral recombination.
  • Nanoparticle-based nonviral vectors enable selective gene delivery with reduced toxicity.

Purpose of the Study:

  • To highlight the fundamental concepts of nonviral gene delivery.
  • To discuss the applications of superparamagnetic iron oxide nanoparticles and functionalized silica nanoparticles in gene delivery.
  • To provide experimental protocols for these nonviral gene delivery systems.

Main Methods:

  • Utilizing functionalized nanoparticles, including superparamagnetic iron oxide and silica nanoparticles.
  • Incorporating genetic materials (plasmid DNA, RNA, siRNA) into nanoparticles.
  • Describing experimental protocols for nanoparticle-based gene delivery.

Main Results:

  • Demonstrated the potential of functionalized nanoparticles for targeted gene delivery.
  • Showcased the advantages of nonviral vectors in terms of safety and versatility.
  • Highlighted the feasibility of using superparamagnetic iron oxide and silica nanoparticles for therapeutic applications.

Conclusions:

  • Nonviral gene delivery using functionalized nanoparticles represents a promising frontier in pharmacotherapy.
  • Superparamagnetic iron oxide and silica nanoparticles are effective nonviral vectors for selective gene targeting.
  • The described methods and protocols facilitate the advancement of nonviral gene delivery research and application.