Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
Transgenic Organisms00:53

Transgenic Organisms

Overview
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combatting ventilator induced diaphragm dysfunction with human bone marrow mesenchymal stromal cell-derived extracellular vesicles.

Skeletal muscle·2026
Same author

Novel molecular assays for monitoring T cell function after HSCT in two siblings with variants in <i>DCLRE1C</i>.

Journal of human immunity·2026
Same author

Three-year follow-up of the COVAXID trial: real-world assessment of SARS-CoV-2 mRNA vaccine immunogenicity in immunocompromised individuals highlights increasing roles of hybrid and passive immunity.

EBioMedicine·2026
Same author

Dynamics of an RNase H-Responsive Tetrahedral DNA Nanostructure for Efficient Intracellular microRNA Inhibition.

Bioconjugate chemistry·2026
Same author

Viral Immunity in Immunoglobulin Products: Global Immunity Debt and Autoimmunity in the Postpandemic Era.

European journal of immunology·2026
Same author

T-cell immunomodulation occurs with different time kinetics during acalabrutinib and zanubrutinib therapy in chronic lymphocytic leukaemia.

British journal of haematology·2025

Related Experiment Video

Updated: Jun 22, 2026

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

Nanotechnology approaches for gene transfer.

Karin E Lundin1, Oscar E Simonson, Pedro M D Moreno

  • 1Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet, Karolinska University Hospital, 141 86 Huddinge, Sweden. karin.lundin@ki.se

Genetica
|June 3, 2009
PubMed
Summary

Nanotechnology offers advanced methods for delivering genetic material into cells for gene therapy and gene silencing. These optimized nucleic acid structures are crucial for precise delivery to cellular compartments like the cytosol or nucleus.

More Related Videos

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

Published on: September 13, 2012

Related Experiment Videos

Last Updated: Jun 22, 2026

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

Published on: September 13, 2012

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Efficiently transferring genetic material into cells is critical for gene therapy and basic research.
  • Targeting specific cellular compartments (cytosol for siRNA, nucleus for transgenes) is essential for desired outcomes.
  • RNA interference (RNAi) has enabled significant advancements in regulating gene expression.

Purpose of the Study:

  • To review nanotechnological approaches for assembling optimized nucleic acid structures.
  • To facilitate targeted gene delivery for therapeutic and research applications.
  • To promote the downregulation of endogenous genes using nanotechnology.

Main Methods:

  • Focus on nanotechnological strategies for nucleic acid derivative assembly.
  • Review of methods for optimizing nanostructures for gene delivery.
  • Exploration of techniques for enhancing gene silencing efficacy.

Main Results:

  • Nanotechnology provides versatile tools for creating precise nucleic acid delivery systems.
  • Optimized nanostructures improve the efficiency and specificity of gene transfer.
  • These approaches enable targeted delivery to specific cellular compartments.

Conclusions:

  • Nanotechnology-based gene delivery systems are vital for advancing gene therapy and gene regulation.
  • Optimized nucleic acid structures are key to successful gene silencing and therapeutic interventions.
  • This review highlights the potential of nanotech for diverse applications in medicine and agriculture.