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Updated: Jun 22, 2026

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
09:49

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

Published on: July 5, 2019

Clinical features of the most common fusion genes in childhood acute lymphoblastic leukemia

J Lazic1, N Tosic, L Dokmanovic

  • 1Department for Hematology and Oncology, University Children's Hospital, Tirsova 10, 11000 Belgrade, Serbia.

Medical Oncology (Northwood, London, England)
|June 3, 2009
PubMed

Insights

This study analyzed genetic translocations in pediatric acute lymphoblastic leukemia (ALL) to understand treatment response. Identifying specific chromosomal aberrations aids in predicting outcomes for children with ALL.

Area of Science:

  • Pediatric Oncology
  • Molecular Genetics
  • Hematology

Background:

  • Current acute lymphoblastic leukemia (ALL) treatments achieve high remission rates but some children remain incurable.
  • Molecular genetic techniques have improved ALL subclassification and minimal residual disease monitoring, crucial for relapse prediction.

Purpose of the Study:

  • To investigate the correlation between specific chromosomal aberrations, clinical features, and treatment response in pediatric de novo ALL.
  • To analyze the incidence and clinical characteristics of ALL subtypes based on major risk-stratifying translocations.

Main Methods:

  • Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect four major translocations (BCR/ABL, MLL/AF4, TEL/AML1, E2A/PBX1).
  • Bone marrow samples from 70 pediatric ALL patients were analyzed at diagnosis, post-induction, and post-intensive chemotherapy.

Main Results:

  • The study identified the presence and incidence of four major risk-stratifying translocations in pediatric ALL.
  • Results provide evidence for variable incidence and distinct clinical characteristics among different ALL subtypes.

Conclusions:

  • Molecular genetic analysis of chromosomal aberrations is vital for understanding ALL heterogeneity.
  • This research contributes to the evidence base for tailoring treatment strategies based on ALL subtypes and genetic profiles.

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