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Related Concept Videos

Trihybrid Crosses02:27

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...

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Related Experiment Video

Updated: Jun 22, 2026

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
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Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

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Optimizing TILLING populations for reverse genetics in Medicago truncatula.

Christine Le Signor1, Vincent Savois, Grégoire Aubert

  • 1Unité Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses à Graines, Institut National de la Recherche Agronomique, Dijon, France.

Plant Biotechnology Journal
|June 4, 2009
PubMed
Summary

Researchers developed two mutant populations in Medicago truncatula for gene function studies. Population 2, generated by single seed descent, showed higher mutation frequency, proving more efficient for genetic screens.

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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

Area of Science:

  • Plant genetics
  • Molecular biology
  • Agricultural science

Background:

  • Medicago truncatula is a key model legume for Vicieae crops.
  • Limited tools hinder systematic gene function investigation in M. truncatula.
  • Developing efficient genetic screening platforms is crucial for legume research.

Purpose of the Study:

  • To create and characterize two mutant populations in M. truncatula.
  • To establish a TILLING platform and phenotypic database for genetic screens.
  • To compare the efficiency of different mutagenesis strategies.

Main Methods:

  • Chemical mutagenesis was applied to M. truncatula to generate two mutant populations.
  • A TILLING platform and phenotypic database were established.
  • Fifty-six targets were screened across both populations for point mutations.

Main Results:

  • A total of 546 point mutations were identified.
  • Population 2 exhibited twice the mutation frequency (1/485 kb) compared to Population 1.
  • The single seed descent strategy (Population 2) was more efficient for mutagenesis density and mutation recovery.

Conclusions:

  • The single seed descent strategy is superior for generating mutant populations in M. truncatula.
  • The developed platform facilitates both reverse and forward genetics screens.
  • Publicly available phenotyping data and a seed ordering tool enhance research accessibility.