Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Guanidine improves DEAE anion exchange-based analytical separation of plasmid DNA.

Electrophoresis·2021

Same author

A tribute to Dr. Serge N. Timasheff, our mentor.

Biophysical reviews·2021

Same author

Removal of empty capsids from adeno-associated virus preparations by multimodal metal affinity chromatography.

Journal of chromatography. A·2021

Same author

Multiple-Monitor HPLC Assays for Rapid Process Development, In-Process Monitoring, and Validation of AAV Production and Purification.

Pharmaceutics·2021

Same author

Excluded Cosolvent in Chromatography.

Journal of pharmaceutical sciences·2018

Same author

A simple and efficient purification platform for monoclonal antibody production based on chromatin-directed cell culture clarification integrated with precipitation and void-exclusion anion exchange chromatography.

Journal of biotechnology·2016

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

Monoclonal antibody purification with hydroxyapatite.

¹Validated Biosystems, 240 Avenida Vista Montana, Ste. 7F, San Clemente, CA 92672, United States. pete@validated.com

New Biotechnology

|June 4, 2009

Summary

Hydroxyapatite (HA) purification effectively removes antibody aggregates, host cell proteins, and leached Protein A. Advanced elution strategies enhance its capability for purifying immunoglobulins and other biologics.

Area of Science:

Biochemistry
Protein Purification
Chromatography

Background:

Hydroxyapatite (HA) has been a cornerstone for immunoglobulin (IgG) purification since the 1950s.
Its applications expanded to IgA and IgM, with detailed understanding of binding mechanisms and ceramic media development.
Recombinant monoclonal antibodies highlighted HA's efficacy in removing aggregates, host cell proteins, and leached Protein A.

Purpose of the Study:

To review the historical and current applications of Hydroxyapatite in protein purification.
To highlight advancements in elution strategies for enhanced purification capabilities.
To discuss the potential of Hydroxyapatite in purifying complex biologics and its future prospects.

Main Methods:

Review of historical data and scientific literature on Hydroxyapatite applications.

More Related Videos

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Published on: September 29, 2011

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Related Experiment Videos

Last Updated: Jun 22, 2026

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Published on: September 29, 2011

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Analysis of binding mechanisms including calcium metal affinity and phosphoryl cation exchange.

Examination of advanced elution strategies and their impact on purification efficiency.

Main Results:

Hydroxyapatite effectively purifies IgG, IgA, and IgM, with significant reduction of antibody aggregates (>60% to <0.1%).
Enhanced purification includes removal of DNA, endotoxin, and virus.
HA demonstrates discrimination of immunological constructs based on variable regions and separation of Fab from Fc fragments.

Conclusions:

Advanced elution strategies have significantly improved Hydroxyapatite's purification performance.
Hydroxyapatite remains a versatile and effective medium for diverse protein purification challenges.
Ongoing developments in HA forms and screening systems promise expanded utility in biopharmaceutical manufacturing.