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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Naturally occurring antisense RNA: function and mechanisms of action
1RNA Biology Group, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle, UK. andreas.werner@ncl.ac.uk
Current Opinion in Nephrology and Hypertension
|June 4, 2009
Summary
Natural antisense transcripts regulate gene expression post-transcriptionally and epigenetically. These transcripts and resulting endo-siRNAs offer new gene silencing strategies for research and clinical applications.
Area of Science:
- Molecular Biology
- Genetics
- Epigenetics
Background:
- Natural antisense transcripts are emerging regulators of gene expression.
- Their transcription influences gene output post-transcriptionally and can establish epigenetic imprints.
Purpose of the Study:
- To review recent advances in understanding natural antisense transcripts.
- To explore their regulatory roles in gene expression and epigenetic modification.
Main Methods:
- Transcriptome sequencing to identify sense-antisense transcript pairs.
- Analysis of regulatory mechanisms including mRNA processing and endo-siRNA formation.
Main Results:
- Widespread expression of complementary sense-antisense transcripts identified.
- Antisense transcripts modulate sense transcript levels and mRNA processing.
- Coexpressed transcripts yield endo-siRNAs, linked to transcriptional silencing.
Conclusions:
- Natural antisense transcripts provide an additional layer of gene regulation.
- Insights into natural antisense transcripts and endo-siRNAs may improve gene silencing strategies.
- Potential applications in biomedical research and clinical settings.
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