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Published on: March 3, 2016
Validated germline-competent embryonic stem cell lines from nonobese diabetic mice
Jennifer Nichols1, Kenneth Jones, Jenny M Phillips
1Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK.
Nature Medicine
|June 4, 2009
Summary
Researchers have derived new embryonic stem (ES) cell lines from Nonobese diabetic (NOD) mice. These cells enable genetic modification and germline transmission, aiding type 1 diabetes research.
Area of Science:
- Immunology
- Genetics
- Developmental Biology
Background:
- Nonobese diabetic (NOD) mice are a key model for studying type 1 diabetes (T1D).
- Dissecting the complex genetic basis of T1D in NOD mice is challenging due to high sequence variation.
- A lack of functional embryonic stem (ES) cells has limited genetic manipulation in NOD mice.
Purpose of the Study:
- To derive and characterize novel NOD ES cell lines.
- To establish a tool for genetic analysis and manipulation in the NOD mouse model.
- To facilitate the study of type 1 diabetes pathogenesis.
Main Methods:
- Derivation of ES cell lines from NOD mouse embryos.
- Genetic modification of NOD ES cells.
- Generation of chimeric mice via ES cell injection.
- Assessment of germline transmission and diabetes development in offspring.
Main Results:
- Successfully derived stable NOD ES cell lines.
- Demonstrated the ability of these ES cells to generate chimeric mice.
- Confirmed efficient germline transmission of modified genes.
- Offspring from germline transmission developed type 1 diabetes.
Conclusions:
- The developed NOD ES cell lines are a valuable resource for type 1 diabetes research.
- These ES cells enable precise genetic dissection of T1D loci.
- Facilitates the creation of new transgenic NOD mouse models for disease study.
