Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimization of a flow cytometry test for routine monitoring of B cell maturation antigen targeted CAR in peripheral blood.

Cytometry. Part B, Clinical cytometry·2024
Same author

Noel Warner Ph.D.

Cytometry. Part B, Clinical cytometry·2023
Same author

Safety and efficacy of tisagenlecleucel in primary CNS lymphoma: a phase 1/2 clinical trial.

Blood·2022
Same author

Editor's response to Bunting et al.

Cytometry. Part B, Clinical cytometry·2021
Same author

From the Editor: Thank you! Remembrances and Issue Highlights.

Cytometry. Part B, Clinical cytometry·2019
Same author

Hematopoietic Stem-Cell Transplantation in the Resource-Limited Setting: Establishing the First Bone Marrow Transplantation Unit in Bangladesh.

Journal of global oncology·2018

Related Experiment Video

Updated: Jun 22, 2026

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
10:20

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Advances in complex multiparameter flow cytometry technology: Applications in stem cell research.

Frederic Preffer1, David Dombkowski

  • 1Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA. preffer@helix.mgh.harvard.edu

Cytometry. Part B, Clinical Cytometry
|June 4, 2009
PubMed
Summary

Recent advances in flow cytometry and cell sorting technology enhance the identification and isolation of rare stem cells. New hardware, reagents, and software improve resolution and specificity for stem cell research.

More Related Videos

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
13:05

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods
10:24

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

Published on: November 19, 2019

Related Experiment Videos

Last Updated: Jun 22, 2026

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
10:20

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
13:05

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods
10:24

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

Published on: November 19, 2019

Area of Science:

  • Biotechnology
  • Cell Biology
  • Immunology

Background:

  • Flow cytometry and cell sorting are essential for stem cell research.
  • Identifying and isolating rare stem cells is a significant challenge.

Purpose of the Study:

  • To review recent technological advancements in flow cytometry and cell sorting.
  • To describe key cell surface and functional markers for stem cell study.

Main Methods:

  • Review of recent improvements in flow cytometric hardware (lasers, optics).
  • Examination of new reagents and software for data visualization.
  • Identification of relevant immunofluorescent and light scatter characteristics.

Main Results:

  • Technological improvements enhance the resolution and specificity of stem cell analysis.
  • New tools allow for more precise identification and isolation of rare stem cells.

Conclusions:

  • Advancements in flow cytometry and cell sorting offer new possibilities for stem cell research.
  • Understanding key antigenic markers is crucial for effective stem cell investigation.