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Updated: Jun 22, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
Modulation filtering enables removal of spikes in fluorescence correlation spectroscopy measurements without
Gustav Persson1, Per Thyberg, Tor Sandén
1Department of Applied Physics, Royal Institute of Technology, Stockholm, Sweden.
Abstract:
The appearance of intensity spikes in measurements is a common problem in fluorescence correlation spectroscopy (FCS) studies of biological samples. In this work, we present a new method for generating artifact-free correlation curves from fluorescence traces that have undergone spike removal. This method preserves the temporal information throughout the measurement and properly represents the correlation between events separated by removed spikes. The method was validated using experimental data. The proposed algorithm is demonstrated herein to be generally applicable, but it is particularly powerful for cases where spikes occur frequently.
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