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Related Experiment Video

Updated: Jun 22, 2026

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
08:59

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Published on: October 5, 2016

Blastocyst microinjection automation.

Leonardo S Mattos1, Edward Grant, Randy Thresher

  • 1Department of Advanced Robotics, Italian Institute of Technology, Genoa 16163, Italy. leonardo.demattos@iit.it

IEEE Transactions on Information Technology in Biomedicine : a Publication of the IEEE Engineering in Medicine and Biology Society
|June 5, 2009
PubMed
Summary

Automating blastocyst microinjection systems enhances efficiency in creating genetically modified mice. This new automated system achieved a 75% success rate for microinjections, improving research productivity.

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Area of Science:

  • Biotechnology
  • Developmental Biology
  • Genetics

Background:

  • Blastocyst microinjection is crucial for generating genetically modified mice in research.
  • Current methods rely heavily on skilled operators, leading to high training costs and time investment.
  • Increased demand for mutant animals in genetic research necessitates improved efficiency.

Purpose of the Study:

  • To automate a previously developed teleoperated blastocyst microinjection system.
  • To enhance productivity and efficiency in the creation of genetically modified mice.
  • To reduce reliance on operator skill and training time.

Main Methods:

  • Designed a new system setup to facilitate automation.
  • Defined rules for automatic microinjections.

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Last Updated: Jun 22, 2026

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
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Published on: October 5, 2016

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Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

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11:40

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  • Implemented video processing for image feedback and developed control algorithms for automation.

    • Main Results:

    • Achieved a 75% microinjection success rate with operator assistance during cell delivery.
    • Resulted in a 53% birth rate from successfully injected blastocysts.
    • Yielded a 20% rate of chimeras, demonstrating successful automation of key steps.

    Conclusions:

    • The developed system successfully automates critical steps of blastocyst microinjection, including penetration and retraction.
    • This automation represents significant progress towards fully automated generation of genetically modified mice.
    • The system shows potential to increase throughput and reduce costs in biomedical research.